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目的:探讨人乳头状病毒(HPV)18,31,45E73个高危基因亚型在新疆哈萨克族(哈族)食管癌中的感染率及其相关性.方法:采用聚合酶链反应(PCR)技术检测HPV-18,31,45E7基因在316例哈族食管癌中的感染情况.并随机抽取PCR产物进行测序来验证PCR结果.结果:HPV18,31和45E7基因亚型在食管癌中阳性率分别为25.3%(80/316),14.2%(45/316)和7.3%(23/316),其基因型分布与年龄、性别均无关.HPV31,45E7基因阳性率在组织病理学分级上无统计学意义.HPV18基因型分布在中低分化食管鳞状细胞癌中的检出率明显高于高分化食管鳞癌(29.6%vs17.7%,χ2=5.398,P<0.05).结论:HPV18型是新疆哈族食管癌的主要高危亚型之一,其基因型分布与食管癌分化程度有关;HPV31,45亚型可能不是新疆哈族食管癌最主要的高危亚型.
Objective: To investigate the prevalence of 18,31,45 E73 high-risk genotypes of human papillomavirus (HPV) in esophageal cancer of the Kazak (Kazakh) ethnic group in Xinjiang and their correlation.Methods: Polymerase chain reaction (PCR) The detection of HPV-18,31,45E7 gene in 316 cases of Ha ethnic esophageal cancer infection, and randomly selected PCR products were sequenced to verify the PCR results.Results: The positive rates of HPV18, 31 and 45E7 subtypes in esophageal cancer were Were 25.3% (80/316), 14.2% (45/316) and 7.3% (23/316), respectively.The genotypes of the two groups were not related to age and sex.The positive rates of HPV31 and 45E7 genes were not statistically significant in histopathological grade (29.6% vs17.7%, χ2 = 5.398, P <0.05) .Conclusion: The HPV18 genotype distribution in moderate-poorly differentiated esophageal squamous cell carcinoma was significantly higher than that in well-differentiated esophageal squamous cell carcinoma Is one of the major high risk subtypes of Ha nationality esophageal cancer in Xinjiang, and its genotype distribution is related to the degree of esophageal cancer differentiation. HPV31,45 subtype may not be the most important high risk subtype of Xinjiang Ha ethnic esophageal cancer.