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胱氨酸、半胱氨酸在汞电极上的极谱行为研究较少。Kolthoff等用直流极谱法研究了Cu~(2+)—NH_3—NH_4Cl溶液中胱氨酸、半胱氨酸的极谱行为,得到了铜络合物的扩散波。U·Forsman用脉冲阴极溶出伏安法研究了胱氨酸及半胱氨酸与铜的络合物的极谱行为。我们用导数示波极谱法,发现在Cu~(2+)—NH_3—NH_4Cl溶液中,当有微量胱氨酸存在时,在-0.76伏处能产生一个灵敏的极谱吸附波,半胱氨酸在同样的条件下也可产生相同的极谱波,利用此极谱波可测定微量胱氨酸或半胱氨酸,检测下限为1×10~(-7)M(胱氨酸)及
There are few studies on the polarographic behavior of cystine and cysteine on mercury electrode. Kolthoff et al. Studied the polarographic behavior of cystine and cysteine in Cu ~ (2 +) - NH_3-NH_4Cl solution by direct current polarography and obtained the diffusion wave of copper complex. U · Forsman pulsed cathodic stripping voltammetry of cystine and cysteine and copper complex polarographic behavior. Using derivative oscillometric polarography, we found that a sensitive polarographic adsorption wave can be produced at -0.76 V in the presence of trace amounts of cystine in Cu ~ (2 +) - NH_3-NH_4Cl solution, The same polarographic wave can be generated under the same conditions. The trace of cystine or cysteine can be determined by this polarographic wave. The detection limit is 1 × 10 -7 M (cystine) and