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Hyrcanoside(Ⅰ)对KB和P-366细胞有抗白血病作用,近来发现它是强心剂,比地高辛毒性稍低。流动注入分析(FIA)是制剂领域广泛使用的工具,方法简单,精密,快速,有多用性。本文介绍用流动注入分光光度法测定Ⅰ,它在碱性介质中同苦味酸反应,生成有色产物,在486 nm处进行测定。作者用本法测定片剂中Ⅰ含量的均匀度,还研究了本法的最佳条件,注入体积为90μl,流速为0.6ml/min,反馈线圈为1.5 m,总分散度D=5.7,样品速率为60/h。Ⅰ在水中稳定。浓度在10～400μg/ml范围内校正曲线呈直
Hyrcanoside (I) has an anti-leukemic effect on KB and P-366 cells and has recently been found to be a cardiotonic agent with slightly less toxicity than digoxin. Flow Injection Analysis (FIA) is a widely used tool in the formulation field. The method is simple, precise, rapid and versatile. This article describes flow injection spectrophotometry for the determination of I, which reacts with picric acid in an alkaline medium to produce a colored product, which is measured at 486 nm. The authors used this method to determine the uniformity of the content of I in tablets. The optimal conditions of this method were also studied. The injection volume was 90μl, the flow rate was 0.6ml / min, the feedback coil was 1.5m, the total dispersion was D = 5.7, The rate is 60 / h. Ⅰ is stable in water. The calibration curve was linear at concentrations ranging from 10 to 400 μg / ml