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本文主要介绍用浓硫酸提取有机氯农药。由于人体脂肪组织样品来自尸体检查和外科手术治疗,这些样品已被严重污染,因此本法的回收率是用奶油脂肪来测定的。匀化的脂肪样品用足量的无水Na_2SO_4捣碎,呈粗粉末状,样品转移到色谱柱中,残留物用150 ml石油醚(b.p.40～50℃)洗脱,洗脱液在38～40℃的旋转蒸发器中蒸发,使脂肪浓度浓缩到约500 mg/ml,再用重量法测定浓缩提取物中脂肪的含量。将1份含有≤500 mg的脂肪样品转移到10 ml磨口塞校准管中,再用石油醚调节体积至10 ml,然后加入0.5 ml浓硫酸,塞紧管塞,
This article describes the use of concentrated sulfuric acid extraction of organochlorine pesticides. Since human adipose tissue samples come from autopsy and surgical procedures, these samples have been heavily contaminated and therefore the recovery of this method is determined using cream fat. Homogenized fat samples were mashed with sufficient amount of anhydrous Na 2 SO 4 to obtain a coarse powder. The sample was transferred to a chromatographic column and the residue was eluted with 150 ml petroleum ether (bp 40-50 ° C) 40 ° C in a rotary evaporator, concentrating the fat concentration to about 500 mg / ml, and determining the amount of fat in the concentrated extract by gravimetry. Transfer 1 portion of fat sample containing ≤ 500 mg into a 10 ml grind calibration tube, adjust the volume to 10 ml with petroleum ether, add 0.5 ml of concentrated sulfuric acid,