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哺乳动物ES细胞的分离与克隆，从一开始就与小鼠胚胎成纤维细胞（MEF）紧密地联系在一起（Evanse和Kaufman，1981）。Meinecke－Tillmann（1996）、尚克刚（1994）等认为，来源不同的成纤维细胞对ES细胞分离效果又有一定影响。本研究利用妊娠终止胚胎，参照前人在小鼠及其它动?
Isolation and cloning of mammalian ES cells has been closely linked to mouse embryonic fibroblasts (MEF) from the outset (Evanse and Kaufman, 1981). Meinecke-Tillmann (1996), Shankar (1994), etc., that derived from different fibroblasts on the ES cell isolation effect to some extent. In this study, the termination of pregnancy embryos, with reference to the previous in mice and other moving?