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目的: 研究和田地区洛浦县长寿与非长寿家族的DNA断裂水平的基础值及其相关因素。方法: 在征得调查对象同意后采集血液样本,用彗星实验方法检测其血淋巴细胞DNA断裂水平。统计分析采用 t检验、秩和检验和相关分析等方法。结果: 长寿与非长寿家族的 DNA断裂率及其校正均数差异都有统计学意义(P <0.01),断裂分级之间差异也有统计学意义(P<0.01),且断裂率与年龄呈正相关,有随年龄增高而增加的趋势,20~39岁组、40~59岁组及60~79岁组之间差异有统计学意义,但是性别之间比较差异无统计学意义(P>0.05)。结论:长寿与非长寿家族的DNA断裂率均数的基础值在排除年龄、性别、吸烟、饮酒等因素的影响后差异仍有统计学意义,长寿与非长寿家族寿命的长短可能与遗传背景产生的DNA损伤不同有一定的关系。
OBJECTIVE: To study the basic DNA fragmentation and its related factors of longevity and non-longevity families in Luopu County, Hotan Prefecture. Methods: Blood samples were collected after the consent of the respondents, and DNA fragmentation of hemolymph cells was detected by comet assay. Statistical analysis using t test, rank sum test and correlation analysis and other methods. Results: There was a significant difference in DNA breakage rate and corrected mean DNA loss between longevity and non-longevity families (P <0.01), and there was also a significant difference between fracture grades (P <0.01). The fracture rate was positively correlated with age (P> 0.05). There was a trend of increasing with age. There was a significant difference between 20 ~ 39 years old group, 40 ~ 59 years old group and 60 ~ 79 years old group, but there was no significant difference between sexes (P> 0.05) . CONCLUSION: The basic values of the DNA breakage rates of longevity and non-longevity families are still statistically significant after exclusion of age, sex, smoking, alcohol consumption and other factors. The longevity and non-longevity of the family life span may be related to the genetic background Different DNA damage has a certain relationship.