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目的:建立同时测定续断中川续断皂苷Ⅵ、马钱苷、当药苷3种活性成分含量的方法,并建立续断药材HPLC特征图谱。方法:采用Thermo BDS Hypersil C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长220 nm。结果:不同产地10批续断样品的HPLC谱图具有很好的一致性,标定了12个共有峰;地理位置相邻的鄂西南、湘西北共有8批样品的HPLC图谱相似度大于0.94。各样品的HPLC指纹图谱相似度结果与3种活性成分总量聚类分析结果一致。结论:所建立的HPLC特征图谱和含量测定方法能较好地评价和控制续断药材的质量,从指纹图谱化学成分信息的角度验证了包括五峰在内的鄂西南(包括临近的湘西北地区)所产续断的道地性或优质性。
OBJECTIVE: To establish a method for the simultaneous determination of three active ingredients in Dipsacus chuanchuan Dosa saponin Ⅵ, loganin, and when glycosides. Methods: The column was eluted with a gradient of acetonitrile - 0.1% phosphoric acid solution at a flow rate of 1.0 mL / min on a Thermo BDS Hypersil C 18 column (250 mm × 4.6 mm, 5 μm) 220 nm. Results: The HPLC chromatograms of 10 samples from different producing areas had good consistency and 12 common peaks were calibrated. The similarity of HPLC chromatograms of 8 batches of samples which were located in the adjacent areas of southwest and northwestern Hunan were greater than 0.94. The similarity of HPLC fingerprints between the samples was consistent with that of the total amount of the three active ingredients. Conclusion: The established HPLC characteristic map and content determination method can evaluate and control the quality of severed medicinal materials better. From the perspective of the chemical composition information of fingerprint, the five southwest of Hubei Province (including the adjacent northwestern Hunan Province) Produced discontinuous authenticity or quality.