论文部分内容阅读
自1920年在厄瓜多尔发现首例人体利什曼病以来,相继有不少报道,但仅基于临床表现和流行病学特点,病原均认为是巴西利什曼种团。最近用同工酶电泳及单克隆抗体对厄瓜多尔6株利什曼原虫分离物鉴定,结果发现:引起人体皮肤损害的3株原虫为巴西利什曼原虫巴拿马亚种,其余从野生动物分离的为墨西哥利什曼原虫亚马逊亚种。本文报告1986年旱季(7～8月)用皮内试验(ST)和ELISA对皮肤损害的患者、居民和学龄儿童共212人的调查结果。 ST的抗原为巴西利什曼原虫前鞭毛体可溶性抗原。蛋白浓度为250μg/ml,并经
Since the first case of human leishmaniasis was discovered in Ecuador in 1920, there have been many reports in succession, but only on the basis of clinical manifestations and epidemiological characteristics, the pathogens are considered as the Brazilian Leishmania. Recently isozyme electrophoresis and monoclonal antibodies to Leishmania isolates from 6 Ecuador were identified and found that the three human protozoan skin lesions caused by Leishmaniasis Panama pancrea, the rest of the animals isolated from Leymus mexican Amazon subspecies. This paper reports the results of a survey of 212 patients with skin lesions, residents and school-age children using the intradermal test (ST) and ELISA during the 1986 dry season (July-August). The antigen of ST is a soluble antigen of promastigotes of Leishmania braziliensis. Protein concentration of 250μg / ml, and by