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目的比较微量板法与试管法检测ABO血型Ig M抗体效价的差异。方法使用微柱凝集法对129例健康供者和91例造血干细胞移植患者进行ABO血型正反定型鉴定,根据反定型结果,同1标本同时经试管法与微量板法进行ABO血型Ig M抗体效价测定并进行比较。结果 220例被研究者中,162例标本需要进行抗体效价检测。抗-A/抗-B效价在不同病例群体中,2种方法检测的一致性差异不具有统计学意义(P>0.05);2种方法检测造血干细胞移植患者ABO血型抗体效价、健康供者抗-A效价的差异不具有统计学意义(P>0.05);微量板法测得的健康供者抗-B效价明显低于试管法(17.78±3.02 vs 19.05±2.95,P<0.05)。结论全自动微量板法微量板法可广泛用于造血干细胞移植患者ABO血型Ig M抗体效价的检测,而用于健康供者抗-B效价检测时抗体效价低于试管法,必要时需要增加试管法。
Objective To compare the titer of ABO blood group Ig M antibody between microtiter plate and tube. Methods ABO blood group positive and negative typing were identified in 129 healthy donors and 91 hematopoietic stem cell transplant patients by microcolumn agglutination. According to the reverse typing results, The price was measured and compared. Results Among the 220 studied subjects, 162 samples were tested for antibody titer. Anti-A / anti-B titers There was no significant difference in consistency between the two methods in different case groups (P> 0.05). Two methods were used to detect antibody titer of ABO blood group in patients with hematopoietic stem cell transplantation, (P> 0.05). The anti-B titer of healthy donors measured by microplate method was significantly lower than that of test tube method (17.78 ± 3.02 vs 19.05 ± 2.95, P <0.05) ). Conclusion The automatic microplate microtiter plate method can be widely used in the detection of ABO blood group Ig M antibody titer in patients with hematopoietic stem cell transplantation. When used in healthy donors, the antibody titer of anti-B titer is lower than that of test tube method. If necessary, Need to increase test tube method.