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目的 克隆外源核酸促电离辐射损伤的小鼠肠腺细胞修复的相关基因 .方法 建立 BAL B/ c小鼠电离辐射后给予外源 RNA与生理盐水治疗的 6 ,12 ,及 2 4h,4和 8d的模型 ,采集空肠组织标本后采用消减杂交基础上的 L D- PCR技术 ,获取与受照小鼠肠腺损伤修复相关的基因克隆 ,对其进行全自动测序与 Gene Bank检索 ,新基因递交给基因库 .结果 获取了 90个与肠腺修复相关的基因 ,杂交证实在核酸治疗组与生理盐水治疗组之间呈差异表达 ,其中 18个是新基因 ,Gene Bank接受号为 AF2 40 16 4- 2 40 181.结论 克隆了 18个外源核酸促电离辐射损伤的小鼠肠腺修复相关基因
OBJECTIVE: To clone the genes involved in the repair of mouse gut gland cells induced by exogenous nucleic acid and induce ionizing radiation.Methods The BALB / c mice were treated with exogenous RNA and saline for 6, 12, 24, 4 and 8 days Of the model, the collection of jejunal tissue specimens by subtractive hybridization based on L D-PCR technology to obtain the mouse intestinal gland injury repair related gene cloning, its full-automatic sequencing and Gene Bank search, the new gene delivery to Gene library.Results 90 genes related to intestinal gland repair were obtained and the hybridization was confirmed to be differentially expressed between the nucleic acid therapy group and the saline treatment group, of which 18 were new genes and the Gene Bank accession number was AF2 40 16 4- 2 40 181. Conclusion Cloned 18 exogenous nucleic acids promote ionizing radiation injury in mouse intestinal gland repair related genes