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为陆地棉产量性状有关的分子标记辅助育种奠定理论基础,以高品质中长绒棉品种‘新陆早24号’为父本,转基因抗虫棉常规品种‘鲁棉研28号’和高产、优质棉花新品种‘冀棉516’为母本,构建F2和F2:3分离群体;利用7638对SSR引物对‘鲁棉研28号’和‘新陆早24号’进行多态性进行筛选,共获得225对多态性引物,对238个F2单株DNA扩增获得238个多态性标记位点,其中185个构建了包括44个连锁群,总长为1509.38cM的遗传连锁图谱,标记间的平均距离为8.16cM,覆盖棉花总基因组的33.91%。根据已有图谱的定位结果,40个连锁群与染色体建立联系。利用复合区间作图法定位‘鲁棉研28号’与‘新陆早24号’分离群体F2单株和F2:3家系的衣分和子指性状QTL,其中得到3个衣分和5个子指的QTL;根据定位结果,选择了14对SSR引物,分析‘冀棉516’与‘新陆早24号’的多态性,其中6个标记构建了两个连锁群。1个衣分和1个子指的QTL在2个群体中均检测到,这些共同QTLs为分子标记辅助育种奠定了基础。
Which laid the theoretical foundation for molecular marker-assisted breeding related to the yield traits of Upland cotton. Using the high quality long-staple cashmere variety ’Xinluzao 24’ as the male parent and the conventional transgenic cotton variety ’Lu Mianyan 28’ and high yielding, A new high-quality new cotton variety ’Jimian 516’ was used as the female parent to construct F2 and F2: 3 segregation populations. Sixty-seven SSR primers were used to screen polymorphisms of ’Lu Mianyan 28’ and ’Xinluzao 24’ A total of 225 pairs of polymorphic primers were obtained and 238 polymorphic markers were amplified from 238 F2 individuals. 185 of them constructed a genetic linkage map including 44 linkage groups with a total length of 1509.38cM, The average distance was 8.16cM, covering 33.91% of the total cotton genome. According to the mapping results of the existing maps, 40 linkage groups are linked to chromosomes. The QTL for lint and off-finger traits of F2 individuals and F2: 3 isolates from ’Luliangyan 28’ and ’Xinluzao 24’ were mapped by composite interval mapping, among which 3 lint and 5 sub-fingers According to the mapping results, 14 pairs of SSR primers were selected to analyze the polymorphism of ’Jimian 516’ and ’Xinluzao 24’, of which 6 markers constructed two linkage groups. QTLs of 1 litter and 1 daughter finger were detected in 2 populations, and these common QTLs laid the foundation for molecular marker-assisted breeding.