To improve the comparability of the research results of ecological industry, the ecological footprint is applied to analyze the resource utilization and environmental pollution in various subsystems, taking maize-MSG as a case.Results show that the production process from maize to MSG is a extended process of ecological footprint, and that the ecological footprint of the maize production is the biggest; the extension of ecological footprint is followed by the increase of footprint profit, which means that the extension of production chain is an important method to improve the resources profit; the systems have a big proportion of the indirect energy ecological footprint; the air and water pollution in MSG subsystem is the most serious. At last, it can be identified that ecological footprint is a good method to measure resource utilization and environmental pollution in various subsystems of an integrated ecological industry.