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本文利用自组装法制备了直接吸附和半胱胺修饰自组装超氧化物歧化酶生物传感器。采用镀金压电石英晶体微电极为工作电极、饱和甘汞电极为参比电极、自制石墨电极为对电极的三电极体系和电化学石英晶体微天平(EQCM)技术对监测超氧化物歧化酶(SOD)在裸金电极和半胱胺修饰金电极上自组装成膜吸附的过程。利用循环伏安法对自组装电极的直接电化学行为进行测定,然后利用电流-时间曲线法对超氧阴离子的响应进行了测定。研究了pH和扫描速率对酶电极的峰电流和峰电位的影响。
In this paper, direct adsorption and cysteamine modified self-assembled superoxide dismutase biosensors were prepared by self-assembly method. Using gold-plated piezoelectric quartz crystal microelectrode as working electrode, saturated calomel electrode as reference electrode, self-made graphite electrode as counter electrode and electrochemical quartz crystal microbalance (EQCM) technology, the monitoring of superoxide dismutase SOD) on bare gold electrode and cysteamine modified gold electrode self-assembly membrane adsorption process. The electrochemical behavior of self-assembled electrodes was determined by cyclic voltammetry. Then the response of superoxide anion was measured by current-time curve method. The effect of pH and scan rate on the peak current and the peak potential of the enzyme electrode was investigated.