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应用酸性聚丙烯酰胺凝胶电泳（ＡＰＡＧＥ）、基因组原位杂交和ＲＦＬＰ标记 研究了异源２号和绵阳２６号在醇溶蛋白和ＤＮＡ水平上的差异性。ＡＰＡＧＥ分析表 明，异源 ２ 号和绵阳 ２６号至少有 ９条醇溶蛋白差异带，且异源 ２号含有小麦背景 中１ＲＳ的醇溶蛋白标记位点Ｇ１ｄ１Ｂ３。基因组原位杂交结果证实异源２号含有黑麦 的１ＲＳ，而绵阳２６号则不含黑麦染色质。ＲＦＬＰ分析结果进一步表明，位于第１ 同源群的短臂ＲＦＬＰ探针均能揭示异源２号和绵阳２６号间的多态性，且异源２号 的１ＢＳ特异性限制性片段发生缺失；而位于第１同源群的长臂和其它同源群上的 探针则不能揭示其多态性。这些结果均说明，异源２号含有１ＢＬ．１ＲＳ易位染色体， 而绵阳２６号则不含１ＢＬ．１ＲＳ易位染色体。它们间的遗传多样性可能主要来自 １ＢＬ．１ＲＳ易位染色体。
The differences of gliadin and DNA levels between heterologous 2 and Mianyang 26 were studied using acid polyacrylamide gel electrophoresis (APAGE), genomic in situ hybridization and RFLP markers. APAGE analysis showed that there were at least 9 different bands of gliadin in Heterologous 2 and Mianyang 26, and heterologous 2 contained the gliadin locus G1d1B3 of 1RS in the wheat background. Genomic in situ hybridization confirmed that heterologous # 2 rye-containing 1 RS, while Mianyang No. 26 did not contain rye chromatin. The results of RFLP analysis further showed that the short arm RFLP probe located in the first homologue group could reveal the polymorphism between heterologous 2 and Mianyang 26 and the lack of 1BS specific restriction fragment of heterologous 2; Probes located on the long arm of the first homologue and on other homologues do not reveal their polymorphism. These results indicate that heterologous 2 contains 1BL. 1RS translocation chromosome, while Mianyang 26 does not contain 1BL. 1RS translocation chromosome. The genetic diversity among them may come mainly from 1BL. 1RS translocation chromosome.