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目的:建立同时测定补脾养胃丸中柚皮苷和新橙皮苷含量的方法。方法:采用HPLC法,色谱柱为Eclipse XDB-C18柱(250 mm×4.6 mm,5μm);流动相:乙腈-甲醇-0.1%磷酸(16∶10∶74),流速:1.0 mL/min;柱温:30℃;检测波长:283 nm;进样量:10μL。结果:柚皮苷和新橙皮苷分别在0.0233-0.2792μg(r=0.9998),0.0224-0.2684μg(r=0.9998)范围内呈良好的线性关系,平均回收率为99.3%、99.7%,RSD%分别为1.71%、1.44%。结论:本方法准确度高,重现性好,可用于补脾养胃丸中柚皮苷和新橙皮苷的含量测定。
Objective: To establish a method for simultaneous determination of naringin and neohesperidin in Bupi Yangwei Pills. Methods: The HPLC column was Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-methanol-0.1% phosphoric acid (16:10:74) Temperature: 30 ℃; detection wavelength: 283 nm; injection volume: 10μL. Results: Naringin and neohesperidin showed a good linear relationship in the range of 0.0233-0.2792μg (r = 0.9998) and 0.0224-0.2684μg (r = 0.9998) with the average recoveries of 99.3%, 99.7%, RSD % Respectively 1.71%, 1.44%. Conclusion: The method is accurate, reproducible and can be used for the determination of naringin and neohesperidin in Bupiyangweiwei pill.