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目的:揭示MUC5AC基因在人胃肠道和生殖道粘膜组织中的表达异质性及其特点,为进一步研究其与疾病关系提供正常的形态学基础。方法:应用免疫组织化学和原位杂交方法。结果:MUC5AC基因编码的核粘蛋白及其mRNA主要分布于人正常胃粘膜表面1/3,包括粘膜的上皮层和胃腺的中上部,呈细颗粒状,位于阳性细胞的胞浆内,核周明显,胃窦、胃底的表达无区别。十二指肠、空肠、结肠及宫颈组织内无表达,胆囊粘膜内灶状分布的阳性染色细胞。结论:MUC5AC基因主要在胃和胆囊组织内表达,而小肠、结肠、宫颈无表达,提示MUC5AC基因表达存在组织异质性。
Objective: To reveal the heterogeneity and characteristics of MUC5AC gene in human gastrointestinal tract and genital tract mucosa, and to provide a normal morphological basis for further study of its relationship with disease. Methods: Immunohistochemistry and in situ hybridization were used. Results: MUC5AC gene encoding nuclear mucin and its mRNA mainly distributed in human normal gastric mucosal surface 1/3, including epithelial mucosa and gastric glands in the upper part, was fine granular, located in the cytoplasm of positive cells, perinuclear Obviously, gastric antrum, gastric fundus expression no difference. Duodenum, jejunum, colon and cervical tissue expression, gallbladder mucosa within the focal distribution of staining cells. CONCLUSION: MUC5AC gene is mainly expressed in gastric and gallbladder tissues but not in small intestine, colon and cervix, suggesting that there is tissue heterogeneity in MUC5AC gene expression.