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单核细胞产生的白细胞间介素I(IL-I)是机体应答微生物侵袭的主要介质。本文报告7例年龄32～79岁(平均54岁)初治菌阳肺结核患者的末梢血单核细胞(PBMC)产生 IL-J 和 T 淋巴细胞母细胞化的能力。按 Ficoll-Hypaqne 沉淀法分离末梢血粘连单核细胞与非粘连 T 淋巴细胞,并用小鼠胸腺细胞增殖法检测 IL-I 活性。用 PPD 和 LPS(脂多糖)诱导淋巴细胞母细胞化,并观察经放射性标记的细胞活性。结果:(1)LPS 和 PPD 诱导单核细胞产生的 IL-I活性(U/ml),结核病患者组(分别为56.1±20.0与28.1±7.2)皆比健康对照组(分别为7.3±1.7与9.5±2.9)明显增高(P<0.05)。(2)患者组 PBMC 经
Interleukin-1 (IL-1), produced by monocytes, is the body’s major mediator of microbial invasion. This article reports on the ability of peripheral blood mononuclear cells (PBMCs) from peripheral blood mononuclear cells (PBMCs) from 7 patients aged 32-79 years (mean age 54 years) with initial treatment of positive pulmonary tuberculosis to produce IL-J and T lymphocyte blasts. Peripheral blood mononuclear cells and non-adherent T lymphocytes were isolated by Ficoll-Hypaqne precipitation method and IL-I activity was detected by mouse thymocyte proliferation assay. Lymphocyte blastocytosis was induced with PPD and LPS (lipopolysaccharide) and the activity of radiolabelled cells was observed. Results: (1) IL-1 activity (U / ml) induced by LPS and PPD in monocytes and tuberculosis patients (56.1 ± 20.0 and 28.1 ± 7.2 respectively) were significantly higher than those in healthy controls (7.3 ± 1.7 vs 9.5 ± 2.9) was significantly higher (P <0.05). (2) Patient group PBMC