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【目的】对转基因植物表达质粒 pROP1中含SBR基因P1片段 ( 1 84～ 1 946bp)进行序列测定和分析。【方法】从E .coliDH5α中提取质粒 pROP1 ,用PCR测序试剂盒和DNA自动测序仪检测P1片段的序列 ,并与GenBank上的变形链球菌表面蛋白基因序列比较 ,检测其准确性。【结果】测定了P1片段 5′端 6 1 6个碱基序列和 3′端 46 6个碱基序列 ,其准确性达98%。【结论】PCR扩增的P1片段 5′端和 3′端的DNA序列与变形链球菌表面蛋白 pac基因唾液粘附区相一致 ,为转基因番茄防龋疫苗的研究提供了基础资料。
【Objective】 The P1 fragment (1 84 ~ 1 946 bp) of SBR gene in pROP1 was sequenced and analyzed. 【Method】 The plasmid pROP1 was extracted from E.coli DH5α, and the sequence of P1 fragment was detected by PCR sequencing kit and DNA sequencer. The accuracy of the fragment was also compared with that of Streptococcus mutans on GenBank. 【Result】 The results showed that the 6 1 6 base sequences at the 5 'end and the 46 6 base sequences at the 3' end of the P1 fragment were determined with an accuracy of 98%. 【Conclusion】 The DNA sequences of 5'and 3'end of P1 fragment amplified by PCR are consistent with the salivary adhesion region of pac gene of Streptococcus mutans, which provides the basic information for the study of anti-caries vaccine against transgenic tomato.