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目的：探讨一氧化氮（ＮＯ）在重症急性胆道感染（ＡＣＳＴ）时大鼠肺损伤中的作用。方法：利用ＡＣＳＴ模型，测定其肺组织中亚硝酸盐／硝酸盐（ＮＯ－２／ＮＯ－３）、环－磷酸鸟苷（ｃＧＭＰ）及水含量的变化。结果：肺组织中ＮＯ－２／ＮＯ－３的含量随时间的进展而逐渐增加（Ｐ＜０．０５）；肺组织的水含量也明显增高（Ｐ＜０．０５）；肺组织中ＮＯ－２／ＮＯ－３含量的变化与ｃＧＭＰ含量的变化及肺水量的变化呈正相关关系（Ｐ＜０．００１）。结论：肺组织中ＮＯ含量增加可能是ＡＣＳＴ时肺损伤的因素之一；ＡＣＳＴ时鸟苷酸环化酶－ｃＧＭＰ－蛋白激酶Ｃ系统活性的改变可能是ＮＯ致肺功能障碍的机理之一。
Objective: To investigate the role of nitric oxide (NO) in lung injury in rats with severe acute biliary tract infection (ACST). Methods: The changes of nitrite / nitrate (NO-2 / NO-3), cyclic guanosine monophosphate (cGMP) and water content in lung tissue were determined by ACST model. Results: The content of NO-2 / NO-3 in lung tissue gradually increased with time (P <0.05), and the water content in lung tissue was also significantly increased (P <0.05) 2 / NO-3 content was positively correlated with cGMP content and lung water volume (P <0.001). CONCLUSION: The increase of NO content in lung tissue may be one of the factors of lung injury during ACST. The alteration of activity of guanylate cyclase-cGMP-protein kinase C system during ACST may be one of the mechanisms of NO-induced pulmonary dysfunction.