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目的研究乌鲁木齐地区健康献血者人群中TTV的流行率。方法分别以UTR区引物和ORF1区引物用巢式PCR法检测乌鲁木齐地区健康献血者人群中TTV及TTV第1基因群的流行率。结果乌鲁木齐地区275例健康献血者中TTV的流行率为97.5%,TTV第1基因群的流行率为69.5%,其流行率在性别、民族间无明显差异(P>0.05),而在各年龄段间存在差异(P<0.05);序列分析和进化树分析结果表明,所检测到的阳性样本均为TTV。结论乌鲁木齐地区健康献血者人群中TTV具有较高的流行率;所流行的TTV与世界各个地区所流行的TTV均具有较高的同源性,说明TTV的流行不受种族和地域的限制。
Objective To study the prevalence of TTV in healthy blood donors in Urumqi. Methods The prevalence of TTV and TTV genotype 1 in healthy blood donors in Urumqi were detected by PCR using ORF1 primers and UTR primers respectively. Results The prevalence rate of TTV was 97.5% in 275 healthy blood donors in Urumqi. The prevalence rate of TTV first gene group was 69.5%. There was no significant difference in gender, ethnicity between the two groups (P> 0.05) (P <0.05). The sequence analysis and phylogenetic tree analysis showed that all the positive samples detected were TTV. Conclusion TTV has a high prevalence in healthy blood donors in Urumqi. The prevalence of TTV is highly homologous to the prevalence of TTV in various regions of the world, indicating that the prevalence of TTV is not restricted by race and geographical area.