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用分子生物学方法自淋病奈瑟菌标准分离株中克隆了４．２ｋｂ的隐蔽性质粒，并根据该质粒ＤＮＡ序列，设计引物，建立淋病奈瑟菌ＰＣＲ检测方法。该方法标本处理简单、反应程序优化，可在２．５小时内完成一次检测。对２１株淋病奈瑟菌标本进行检测，结果全为阳性；而对２２株其它菌株检测均为阴性，证实了该法的特异性。与涂片、培养法比较，ＰＣＲ阳性率最高。结合临床检测了７０６例性病患者，总阳性率为２８．６％（２０２／７０６）。实验表明该ＰＣＲ方法简便快速、敏感特异，对淋病的流行病学调查和早期防治有重要意义。所克隆的含有４．２ｋｂ隐蔽性质粒ＤＮＡ还可用作制备探针和ＰＣＲ阳性对照。
A 4.2kb cryptic plasmid was cloned from the standard strain of Neisseria gonorrhoeae by molecular biology method. Based on the DNA sequence of the plasmid, a primer was designed and a PCR assay for Neisseria gonorrhoeae was established. The method of specimen processing is simple, the reaction procedure is optimized, and the test can be completed in 2.5 hours. 21 strains of Neisseria gonorrhoeae were tested, the results were all positive; and 22 strains of other strains were negative, confirmed the specificity of the method. Compared with smear and culture method, PCR positive rate was the highest. In combination with clinical tests of 706 cases of STD patients, the total positive rate was 28.6% (202/706). Experiments show that the PCR method is simple, rapid and sensitive, and it is important for the epidemiological investigation and early prevention and treatment of gonorrhea. The cloned 4.2kb concealed plasmid DNA can also be used as a preparation probe and PCR positive control.