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试验对鸭源新型鹅细小病毒(Duck derived goose parvovirus,DDPV)DS15株在鸭胚上的增殖特性及对鸭胚的致病力进行了研究。结果显示,鸭胚接种DDPV DS15株以后,96~120 h出现死亡,胚体全身出血,病毒滴度ELD50为10-5.5/0.2 m L。以鸭胚增殖的病毒攻击樱桃谷肉鸭,复制出典型的鸭“大舌病”临床症状,并从发病鸭脏器中分离到病毒。对DDPVDS15株VP2基因进行克隆并并将其因序列与国内外报道的部分鹅细小病毒(GPV)及番鸭细小病毒(MDPV)分离株的VP2基因进行比对和分析。结果表明,DS15株VP2基因与GPV之间的核苷酸和氨基酸同源性分别为89.2%~96.5%和93.4%~97.8%,而与MDPV核苷酸和氨基酸同源性则较低,分别为80.1%~89.3%和87.8%~93.5%;在亲缘关系方面,DDPV DS15株与GPV较近,提示二者可能是由同一病毒进化而来。
The proliferation of Duck derived goose parvovirus (DDPV) strain DS15 on duck embryo and its virulence on duck embryos were studied. The results showed that after inoculation of DDPV DS15 strain, duck embryos died after 96-120 h and the whole body of the embryo body blew. The virus titer ELD50 was 10-5.5 / 0.2 m L. The attack by duck embryo virus cherry Valley duck, replicate the typical duck “big tongue disease ” clinical symptoms, and isolated from the diseased duck organism virus. VP2 gene of DDPVDS15 strain was cloned and its sequence was compared with the VP2 gene of some goose parvovirus (GPV) and Muscovy duck parvovirus (MDPV) isolates reported at home and abroad. The results showed that the nucleotide and amino acid homology between VP2 gene and GPV of DS15 strain were 89.2% -96.5% and 93.4% -97.8%, respectively, but lower than those of MDPV nucleotide and amino acid respectively 80.1% -89.3% and 87.8% -93.5%, respectively. In the aspect of genetic relationship, DDPV DS15 strain was closer to GPV, suggesting that both may have evolved from the same virus.