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【目的】研究外源茉莉酸处理玉米地上部(或地下部)是否会系统性地影响到地下部(或地上部)的防御作用,探讨对玉米防御反应的影响是否与玉米的茉莉酸信号途径相关。【方法】以“高油115”玉米为材料,采用化学物质分析和基因表达分析方法,通过茉莉酸合成途径抑制剂水杨苷异羟肟酸(salicylhydroxamic acid,SHAM)预处理的对比实验,研究茉莉酸在玉米地上部与地下部诱导防御反应中的作用。【结果】外源茉莉酸处理玉米地上部能系统地影响到地下部防御物质的含量及防御相关基因的表达,处理地下部也能系统性地影响地上部。茉莉酸处理地上部以及茉莉酸处理地下部对玉米叶片防御反应的诱导均强于根系,而茉莉酸处理地上部对玉米同一部位的诱导作用强于茉莉酸处理地下部。茉莉酸处理地上部是通过激活茉莉酸自身合成来诱导叶片Bx1、Bx9、PAL、PR-1、MPI、FPS和TPS基因的表达、增加叶片丁布含量及降低咖啡酸含量的,同时系统诱导根系Bx6、Bx9、PAL和PR-2a基因表达、增加香豆酸和咖啡酸含量以及降低丁香酸含量则与激活地上部茉莉酸自身合成相关。茉莉酸处理玉米地下部对根系Bx6、PR-2a和MPI基因表达的诱导以及总酚含量的降低也是通过激活茉莉酸自身合成来实现的,对地上部(叶片)防御相关基因Bx6、Bx9、PAL、FPS和TPS表达的间接诱导作用以及丁布、香豆酸、咖啡酸和丁香酸的增加作用则与激活地下部茉莉酸自身合成相关。【结论】外源茉莉酸处理地上部能系统影响到地下部的防御反应,反之亦然;茉莉酸对玉米的诱导防御以叶片为主;茉莉酸处理地上部对玉米的诱导作用强于地下部处理;茉莉酸处理玉米的地上部或地下部,对处理部位防御作用的影响主要是通过激活玉米体内茉莉酸自身合成起作用的;茉莉酸处理玉米对非处理部位防御作用的影响则与激活处理部位茉莉酸自身合成相关。
【Objective】 The purpose of this study was to investigate whether the exogenous jasmonic acid treatment of corn shoots (or underground part) could systematically affect the defense of the underground part (or aboveground part) and explore whether the impact of defense response to maize jasmonic acid signal pathway Related. 【Method】 The experiment of chemical substance analysis and gene expression analysis was conducted on “Gaoyou115 ” maize, and compared with the pretreatment of salicylic acid salicylhydroxamic acid (SHAM), a jasmonic acid synthetic pathway inhibitor , To study the role of jasmonic acid in inducing defensive responses in aerial parts and underground parts of maize. 【Result】 The results showed that the exogenous jasmonic acid treatment of corn shoots could systematically affect the content of defense components and the expression of defense related genes. The treatment of underground parts also affected the shoots systematically. The induction of defense response to maize leaves was stronger than shoots in the shoots of jasmonic acid and underground jasmonic acid treatment, while the shoot induction of jasmonic acid on the same part of corn was stronger than that of jasmonic acid treatment. JA could enhance the expression of Bx1, Bx9, PAL, PR-1, MPI, FPS and TPS genes and increase the content of butyricin and caffeic acid in the aerial part of jasmonic acid by inducing the synthesis of jasmonic acid. Bx6, Bx9, PAL and PR-2a gene expression, increased coumaric acid and caffeic acid content and decreased syringic acid content was associated with the activation of shoots jasmonic acid synthesis. The induction of root Bx6, PR-2a and MPI gene expression and the reduction of total phenolic content in roots of jasmonic acid-treated corn were also achieved through activation of jasmonic acid self-synthesis. The effects of shoot defense-related genes Bx6, Bx9, PAL , Indirect induction of FPS and TPS expression, and the increasing action of butyric acid, coumaric acid, caffeic acid and syringic acid are related to the activation of jasmonic acid itself. 【Conclusion】 The exogenous jasmonic acid could affect the defense responses of the shoots in the shoots and vice versa. The induction and defense of jasmonic acid on maize mainly took the form of leaves. The shoot induction of shoots by jasmonic acid was stronger than that of the ground parts The effect of jasmonic acid treatment on the aboveground or underground parts of corn was mainly due to the activation of jasmonic acid itself in maize plants. The effect of jasmonic acid treatment of maize on the non-treatment parts was related to the activation treatment Parts of jasmonic acid synthesis related to their own.