目的 :纯化HLA DR分子。方法 :纯化抗HLA DR单克隆抗体L2 4 3并与CNBr活化的Sepharose 4B偶联制备亲和柱 ,应用免疫亲和层析方法纯化HLA DR分子。结果 :从 5gHLA DR阳性的人B淋巴瘤细胞系RAJI裂解液中得到 2 0 μgHLA DR分子。它们以二聚体形式存在 ,能与构象依赖型单抗L2 4 3进行结合 ,煮沸变性后分开为α和 β两条单链。这为MHCⅡ类分子抗原表位研究奠定了基础 Purpose: To purify HLA DR molecules. Methods: The anti-HLA DR monoclonal antibody L2 4 3 was purified and coupled with CNBr activated Sepharose 4B to prepare affinity column. The HLA DR molecule was purified by immunoaffinity chromatography. Results: 20 μg of HLA DR molecule were obtained from 5 g of HLA DR positive human B lymphoma cell line RAJI lysate. They exist as dimers and bind to the conformation-dependent mAb L2 4 3, boiled to denature and separated into two single-stranded chains, α and β. This laid the foundation for MHC class II epitope research