论文部分内容阅读
目的:建立RP-HPLC法同时测定苦碟子药材中木犀草素7-O-β-D葡萄糖1→2葡萄糖苷(Ⅰ)、木犀草素-7-O-β-D-葡萄糖苷(Ⅱ)、木犀草素7-O-β-D-葡萄糖醛酸苷(Ⅲ)、芹菜素-7-O-β-D葡萄糖醛酸苷(Ⅳ)的含量。方法:采用C_(18)色谱柱(250mm×4.6 mm,5μm),以乙腈-0.4%磷酸溶液为流动相,梯度洗脱;检测波长348 nm。结果:8批次苦碟子药材中Ⅰ含量在0.30～1.20 mg·g~(-1),Ⅱ含量在0.30～1.10 mg·g~(-1),Ⅲ含量在2.40～6.00 mg·g~(-1),Ⅳ含量在0.90～1.70 mg·g~(-1);平均回收率为Ⅰ:97.32%(RSD=0.91%);Ⅱ:98.87%(RSD=0.86%);Ⅲ:98.55%(RSD=1.62%);Ⅳ:96.40%(RSD=0.85%)。结论:该方法简便,准确,灵敏,可用于苦碟子药材的质量控制。
OBJECTIVE: To establish a RP-HPLC method for the simultaneous determination of luteolin 7-O-β-D glucose 1 → 2 glucoside (Ⅰ), luteolin-7-O-β-D-glucoside (Ⅱ) , Luteolin 7-O-β-D-glucuronide (Ⅲ) and apigenin-7-O-β-glucuronide (Ⅳ) Methods: C_ (18) column (250 mm × 4.6 mm, 5 μm) with acetonitrile-0.4% phosphoric acid as mobile phase was used for gradient elution. The detection wavelength was 348 nm. Results: The content of Ⅰ in 8 batches of Kudiezi was 0.30-1.20 mg · g -1, the content of Ⅱ was 0.30-1.10 mg · g -1, the content of Ⅲ was 2.40-6.00 mg · g -1 -1), the content of Ⅳ was in the range of 0.90-1.70 mg · g -1, the average recovery was 97.32% (RSD = 0.91%), Ⅱ: 98.87% (RSD = 0.86%), Ⅲ: 98.55% RSD = 1.62%); IV: 96.40% (RSD = 0.85%). Conclusion: The method is simple, accurate and sensitive and can be used for the quality control of kudzu medicine.