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目的掌握福建口岸鼠形动物间鼠疫、肾综合征出血热和钩端螺旋体病等传染病的流行情况,为采取相应的防治措施提供依据。方法2010—2012年采用鼠笼法在福建口岸捕获鼠形动物;以间接血凝和反向间接血凝实验对鼠血清和肝脏分别进行鼠疫F1抗体及抗原的检测;以套式RT-PCR对鼠肺进行汉坦病毒核酸检测;以PCR对鼠肾进行钩端螺旋体核酸检测。结果本次监测采集1279份鼠血清及2430份鼠肝脏标本,分别进行鼠疫F1抗体、F1抗原检测,均为阴性;采集的845份鼠肾标本检出钩体核酸阳性29份,阳性率为3.43%;采集的2340份鼠肺标本检出汉坦病毒核酸阳性150份,阳性率为6.41%,均为家鼠型。结论福建口岸鼠疫处于静息状态,鼠形动物传播肾综合征出血热和钩端螺旋体病的风险存在,应加强对主要宿主携带病原体的检测,并针对其流行特点采取措施。
Objective To understand the prevalence of infectious diseases such as plague, hemorrhagic fever with renal syndrome and leptospirosis in Fujian port, and to provide basis for taking corresponding control measures. METHODS: Murine animals were captured in Fujian port by squirrel-cage method from 2010 to 2012. F1 antibody and antigenicity of plague were detected by indirect hemagglutination and indirect indirect hemagglutination test respectively. Rat lung was detected by Hantavirus nucleic acid; PCR was used to detect Leptospira spp. Results 1279 serum samples of rat and 2430 rat liver samples were collected for the detection of F1 antibody and F1 antigen in the plague. All of the 845 kidney samples collected from the mice were positive for leptospire nucleic acid, with a positive rate of 3.43 %; Of the 2340 murine samples collected, 150 were positive for Hantavirus, the positive rate was 6.41%. All of them were domestic mice. Conclusions The plague in Fujian port is in a resting state. There is a risk of the mouse-type animals spreading hemorrhagic fever with renal syndrome and leptospirosis. Therefore, the detection of host-borne pathogens should be strengthened and measures should be taken according to their epidemiological characteristics.