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目的研究儿童临床分离的30株肠球菌,红霉素耐药性在同属同种、同属异种和异属细菌之间的传递。方法使用临床实验室标准化研究所(CLSI)推荐的琼脂稀释法筛选30株耐红霉素的肠球菌,通过滤膜接合法进行质粒接合转移试验,采用 PCR 检测供体菌、受体菌和接合子 ermB基因和转座子 Tn1545、Tn917。结果 13株粪肠球供体菌通过质粒接合转移试验后得到13株接合子,红霉素最小抑菌浓度(MIC)≥512μg/ml,除原供体菌1株粪肠球菌阴性外,其余的12株接合子全部携带 ermB 基因,且 ermB 基因均同时存在于转座子 Tn1545和 Tn917;16株屎肠球供体菌和1株海氏肠球供体菌通过质粒接合转移试验后得到17株接合子,红霉素 MIC≥512μg/ml,除原供体菌1株屎肠球菌阴性外,其余16株接合子全部携带 ermB 基因,并且有11株接合子 ermB 基因同时存在于 Tn1545和 Tn917,4株接合子 ermB 基因仅存在于 Tn1545,1株接合子 ermB 基因仅存在于 Tn917;30株肠球菌通过质粒接合转移试验后得到的30株金黄色葡萄球菌接合子,红霉素 MIC≥512μg/ml,除原供体菌1株粪肠球菌和1株屎肠球菌阴性外,其余的28株接合子全部携带 ermB 基因,其中ermB 基因同时存在于 Tn1545和 Tn917的为23株,4株接合子 ermB 基因仅存在于 Tn1545,1株接合子 ermB 基因仅存在于 Tn917。结论肠球菌对红霉素的耐药性可以在同属同种、同属异种和异属之间进行传递;红霉素的耐药性与 ermB 基因存在一致,ermB 基因与 Tn1545和 Tn917密切相关,ermB 基因与 Tn1545和 Tn917可以在同属同种、同属异种和异属之间进行传递。
Objective To study the clinical isolates of 30 enterococci in children, erythromycin resistance in the same species, belong to the same species and heterologous bacteria transmission. Methods Thirty erythromycin-resistant enterococci were screened by the CLSI recommended agar dilution method. The conjugation and transfer of plasmids were carried out by the membrane-bound method. The donor and recipient bacteria and the conjugates Sub-ermB gene and transposon Tn1545, Tn917. Results Thirteen strains of conjugates were obtained from 13 strains of Enterococcus faecalis donors. The minimum inhibitory concentration (MIC) of erythromycin was above 512 μg / ml. Except that one strain of Enterococcus faecalis was negative, Of the 12 zygotes all carry ermB gene, and ermB gene both exist in the transposon Tn1545 and Tn917; 16 feces enterococcal donor bacteria and a strain of Haematobio colon donor transfer by plasmid conjugation transfer test 17 Strain zygote, erythromycin MIC ≥ 512μg / ml, in addition to the original donor strains of Enterococcus faecium negative, the other 16 strains all carry ermB gene, and 11 zygotes ermB gene exists in both Tn1545 and Tn917 , Four clones ermB gene only exists in Tn1545, a strain ermB gene exists only in Tn917; 30 Enterococcus faecalis by plasmid conjugation transfer test obtained 30 Staphylococcus aureus zygotes, erythromycin MIC ≥ 512μg / ml. Except for a strain of Enterococcus faecalis and one strain of Enterococcus faecium negative for the original donor bacteria, the remaining 28 strains all carried ermB gene. Among them, 23 genes with ermB gene in Tn1545 and Tn917, 4 strains The sub-ermB gene exists only in Tn1545 and the first inbred ermB Gene exists only in Tn917. Conclusion The resistance of Enterococcus to erythromycin can be transmitted by the same species, the same species and the same genus. The resistance of erythromycin is consistent with that of ermB gene. The ermB gene is closely related to Tn1545 and Tn917. The expression of ermB Gene and Tn1545 and Tn917 can be in the same species, belong to the same species and transmission between different genus.