论文部分内容阅读
目的 :本研究旨在观察机动车尾气对大鼠气道高反应性的影响。方法 :取30只SD大鼠,随机分5组:正常对照组;吸入尾气2天组;吸入尾气4天组;吸入尾气6天组;吸入尾气8天组,每组6只。采用小动物呼吸机,肺组织切片PAS和HE染色,检测各组大鼠呼吸道反应性,肺组织杯状细胞化生指数、上皮粘液储备指数和炎症细胞数量。结果 :随着尾气吸入时间的延长,大鼠出现越来越明显的气道高反应性,同时,呼吸道粘液分泌量显著增加,气道炎症明显加重。结论 :吸入机动车尾气能引发并加重气道高反应性。
Objective: This study aimed to observe the influence of vehicle exhaust on airway hyperresponsiveness in rats. Methods: Thirty SD rats were randomly divided into 5 groups: normal control group, inhalation of exhaust gas for 2 days, inhalation of exhaust gas for 4 days, inhalation of exhaust for 6 days, inhalation of exhaust for 8 days with 6 rats in each group. Small animal respirator and lung tissue sections were stained with PAS and HE for detecting respiratory reactivity, goblet cell goblet cells index, epithelial mucus reserve index and inflammatory cells in each group. Results: With the prolongation of tail gas inhalation, the rats showed more and more obvious airway hyperresponsiveness. At the same time, the airway mucus secretion increased significantly and the airway inflammation increased obviously. Conclusion: Inhaling motor vehicle exhaust can induce and aggravate airway hyperresponsiveness.