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肿癌坏死因子(TNF)分为α、β、γ三型,分别由巨噬细胞、淋巴细胞和NK细胞产生。TNF除了能使某些肿瘤细胞在体内引起出血性坏死和在体外被直接杀伤外,还有许多其它生物学活性。很多学者认为TNF也是急、慢性感染过程中主要的细胞因子介质。给动物注入人的重组TNF(r-TNF)酷似注内毒素或严重革兰氏阴性菌感染后的反应。但危重烧伤病人与TNF的关系在国内尚未见报导。 作者应用双抗体夹心型ELISA微量反应板法测定43例正常人及动态观察19例重症烧伤病人(TBSA 30～85％)的TNF水平。在应用本法测定中,第一抗体为鼠抗人TNF的单克隆抗体,并以r-TNF为标准(单抗及r-TNF为军事医学科学院基础所分子免疫室沈倍奋教授惠赠)。43例正常人中,TNF有20例测不出,其余23例为0.0055±0.0018μg/ml。
Tumor necrosis factor (TNF) is divided into α, β, γ three types, respectively, by macrophages, lymphocytes and NK cells. In addition to enabling certain tumor cells to cause hemorrhagic necrosis in vivo and direct killing in vitro, TNF has many other biological activities. Many scholars believe that TNF is also a major cytokine mediator during acute and chronic infection. Recombinant TNF (r-TNF) injected into animals resembles reactions following endotoxin injection or severe Gram-negative bacterial infections. However, the relationship between patients with critical burn and TNF has not been reported in China. The authors used double-antibody sandwich-type ELISA microdialysis plate method to determine the level of TNF in 43 normal subjects and in 19 severe burn patients (TBSA 30-85%). In the application of this assay, the first antibody is a mouse anti-human monoclonal antibody to TNF, and r-TNF as the standard (monoclonal antibody and r-TNF for the Academy of Military Medical Sciences, based on the Department of Molecular Immunology Professor Shen Bei Fen gift). Twenty-three of the 43 normal subjects were undetectable and the other 23 were 0.0055 ± 0.0018 μg / ml.