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1977年我们进行甘蔗体细胞育苗的探索性试验。经过两年多时间的实验研究,现在已经能用胚状体繁殖出数以万计的蔗苗,采用广口瓶和大三角瓶等大容器培养的蔗苗(封三图1),已经应用于快速繁殖甘蔗优良品种。到目前为止,已经从8个甘蔗品种的体细胞诱导形成胚状体再产生完整植株,并且筛选出它们的胚性细胞团,建立了“1号、8号、9号”3个胚性细胞无性繁殖系,经过一年零十个月内17次继代培养,仍能保持旺盛的繁殖能力和正常分化植株。
In 1977 we conducted sugarcane somatic cell seedling exploratory test. After more than two years of experimental research, it is now possible to breed tens of thousands of sugarcane plants with embryoid bodies, using sugarcane seedlings grown in large containers such as jars and big triangle bottles In the rapid propagation of sugarcane varieties. To date, whole plants have been induced from somatic cells of eight sugarcane varieties to form embryoid bodies, and their embryogenic cell mass has been screened to establish three embryogenic cells, “No. 1, No. 8 and No. 9” Asexual propagation line, after 17 subcultures within 10 months of the year, can still maintain strong reproductive capacity and normal differentiation of plants.