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目的：从mRNA和蛋白水平检测斯钙素-1（STC-1）在乳腺癌细胞系及乳腺癌患者外周血中的表达，并探讨其临床意义。方法2012年7月至2013年1月期间，应用RT-PCR检测3个乳腺癌细胞系、沈阳军区总医院收治的56例乳腺癌患者及10例健康女性外周血STC-1 mRNA的表达；应用酶联免疫吸附实验法检测STC-1蛋白的表达。结果在mRNA和蛋白水平，乳腺癌细胞系及患者外周血中STC-1表达均明显高于健康女性；且在不同TNM分期表达有显著差异（P<0.05），Ⅳ期显著高于Ⅰ、Ⅱ、Ⅲ期（P5 ng/ml时表达较CEA≤5 ng/ml时明显增高（P<0.05）。结论 STC-1有望成为乳腺癌复发转移的标志物，其表达水平对于乳腺癌预后预测具有重要价值。“,”Objective To detect the expression of STC-1 mRNA and protein levels in cell lines and patients of breast cancer, and to investigate the clinical significance. Methods The reverse transcription polymerase chain reaction (RT-PCR) was used to detect STC-1mRNA in 3 breast cancer cell lines, the peripheral blood samples of 56 patients with breast cancer and 10 healthy women. The enzyme linked immunosorbent assay (ELISA) was used to detect STC-1 protein levels in supernatant of breast cancer cell lines, and in serum of 56 patients as well as 10 healthy women. Results The expression of STC-1 of breast cancer cells and patients with breast cancer were statistically higher than that of the healthy women in both mRNA and protein levels (P<0.05). The mRNA and protein levels of STC-1 in patients with breast cancer were related to the TNM stage (P<0.05), both were higher in stageⅣthan in stageⅠ,Ⅱ, andⅢ(P5 ng/ml than those when CEA≤5ng/ml(P<0.05). Conclusion STC-1 could be used as markers of recurrence and metastasis of breast cancer, and might be of great significance in judging prognosis.