目的　构建人BLyS基因真核细胞表达载体。方法　采用RT PCR方法 ,从激活的人外周血淋巴细胞的总cDNA中扩增得到 876bp的人BLyScDNA片段 ,再用XhoⅠ和EcoRⅠ双酶切后定向克隆到真核细胞表达载体pcDNA3中 ,用限制性内切酶酶切分析和DNA序列分析鉴定重组质粒。结果　人BLyScDNA已经正确克隆到真核细胞表达载体pcDNA3中。结论　本实验结果为研制抗人BLyS单克隆抗体来研究BLyS与B淋巴细胞功能的关系以及进一步探索自身免疫性疾病的治疗新途径奠定了基础 Objective To construct eukaryotic expression vector of human BLyS gene. Methods The BLyScDNA fragment of 876bp was amplified by RT-PCR from the total cDNA of activated human peripheral blood lymphocytes. The recombinant plasmid was digested with XhoⅠ and EcoRⅠ and cloned into eukaryotic expression vector pcDNA3. Endonuclease digestion analysis and DNA sequence analysis identified the recombinant plasmid. Results Human BLyScDNA has been correctly cloned into the eukaryotic expression vector pcDNA3. Conclusions Our results lay the foundation for the development of monoclonal anti-human BLyS antibodies to study the relationship between BLyS and B-lymphocyte function and to further explore new avenues of treatment for autoimmune diseases