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报道了一种基于等离子体聚合膜、结合聚电解质设计的转铁蛋白压电免疫传感器 .采用辉光放电等离子体聚合技术 ,在石英晶体上沉积一层正丁胺聚合膜 ,再在膜上自组装一层易再生的、带负电的聚电解质 ,调节抗体溶液的 p H值使其带正电 ,经静电吸附包被抗体后用以测定抗原 .探讨了自组装聚电解质的浓度和自组装时间 ,抗体的包被浓度、包被时间和 p H值以及免疫反应的酸度、温度及响应频率与时间的关系等实验条件的影响 .考察了传感器的灵敏度、选择性、重现性和再生性能 .用传感器测定人血清中转铁蛋白的线性范围为 0 .1 0～ 1 2 .65μg/ m L.将其用于实际样品中转铁蛋白的测定 ,结果与酶联免疫法基本一致 .
A kind of transferrin piezoelectricity immunosensor based on plasma polymerization membrane and polyelectrolyte was designed.It was deposited on the quartz crystal by glow discharge plasma polymerization Assemble a layer of renewable, negatively charged polyelectrolytes, adjust the p H value of the antibody solution to make it positively charged, and coat the antibody for detection of the antigen after electrostatic adsorption. The concentration of self-assembled polyelectrolyte and self-assembly time , The concentration of coating, the coating time and p H value, the acidity and temperature of the reaction, the relationship between the response frequency and time, etc. The sensitivity, selectivity, repeatability and reproducibility of the sensor were investigated. The linear range of transferrin in human serum measured by the sensor was 0. 110 ~ 1.265μg / m L. It was applied to the determination of transferrin in real samples, and the results were basically consistent with enzyme-linked immunosorbent assay.