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目的 :观察益气养阴方诱导急性白血病模型小鼠细胞凋亡的作用 ,探讨益气养阴方治疗急性白血病的作用机制。方法 :以L72 12 白血病小鼠为研究对象 ,用益气养阴方浓缩液给小鼠灌胃 8d后处死 ,利用光学显微镜、DNA琼脂糖凝胶电泳等手段观察益气养阴方对急性白血病的作用。结果 :形态学观察可见细胞凋亡特征 ,DNA琼脂糖凝胶电泳图谱显示细胞凋亡现象。结论 :本研究提示诱导白血病细胞凋亡是益气养阴方治疗急性白血病的重要机制之一
Objective: To observe the effect of Yiqi Yangyin Recipe on inducing apoptosis of acute leukemia model mice and to explore the mechanism of Yiqi Yangyin Recipe in treating acute leukemia. Methods: L72 12 leukemia mice were used as research object. The mice were inoculated with Yiqi Yangyin Concentrate for 8 days and then sacrificed. Using light microscope and DNA agarose gel electrophoresis, the effects of Yiqi Yangyin prescription on acute leukemia effect. Results: Apoptotic characteristics were observed by morphological observation. DNA agarose gel electrophoresis showed apoptosis. Conclusion: This study suggests that induction of apoptosis in leukemia cells is one of the important mechanisms of Yiqi Yangyin prescription in the treatment of acute leukemia