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在小麦叶枕距±2cm时，喷施4种不同化学杂交剂（CHA）后，分别取小孢子处于不同发育阶段的花药，进行过氧化酶（POD）、淀粉酶（Amy）和酯酶（Est）等同工酶的分析。研究表明：在小孢子母细胞减数分裂期，处理2、3和4三种CHA对POD的A1、A2；B2、B4和B5，C1、C2和C3同工酶带的活性均有明显的抑制作用；处理5除去对A1和A2表现抑制外，对其他酶带的活性均有增强作用。在单枚早期，处理2和3的A、B和C区POD同工酶活性均明显低于对照；处理4和5上述各区POD同工酶活性却明显高于对照。在上述两个发育时期，处理2对Amy1区酶活性有增强作用，而处理3、4和5对该区酶活性却表现了专一性的抑制。各处理对Est同工酶A区和B区的酶活性主要表现为抑制。实验结果表明，不同的CHA均通过干扰花药的物质和能量代谢而导致雄性生理性不育。
Four different chemical hybridization agents (CHA) were sprayed on wheat leaves at a distance of ± 2cm from the anthers of microspores at different developmental stages. Peroxidase (POD), amylase (Amy) and esterase Est) isoenzyme analysis. The results showed that the activities of isozyme bands A1, A2; B2, B4 and B5, C1, C2 and C3 of POD were all significantly different at the meiosis stage of microspore mother cells Inhibition; treatment 5 to remove the inhibition of A1 and A2, the activity of other enzyme bands have enhanced role. POD isozymes in treatments A, B and C of treatments 2 and 3 were significantly lower than controls at the early stage of treatment. POD isozymes of treatments 4 and 5 above were significantly higher than those of the control. In the two developmental stages, treatment 2 enhanced the activity of Amy1, and treatments 3, 4 and 5 showed specific inhibition of the activity of this area. The enzyme activity of Est isozymes A and B in each treatment was mainly inhibited. The experimental results show that different CHA can cause male physiological infertility by interfering with the anther’s material and energy metabolism.