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布鲁菌病是一种由布鲁菌属感染引起的危害严重的人兽共患病。经典的布鲁菌分型方法将其分为6个生物种22个生物型,DNA-DNA杂交研究显示6个经典物种同源性>90%。多位点可变数目串联重复序列分析(MLVA)依据可变数目串联重复序列(VNTR)特征进行菌株基因分型,在血清分型的基础上进一步分型,阐明了环境菌株和临床菌株的关系,并在布鲁菌病暴发流行调查中得到了广泛应用。该文综述了MLVA技术基本原理、发展概况、优越性及其在布鲁菌分型和溯源中的应用。
Brucellosis is a zoonosis that is caused by the infection of Brucella. The classical Brucella typing method divides it into 22 biotypes of 6 biotypes, and DNA-DNA hybridization studies show> 90% homology among the 6 classical species. Multisite variable number tandem repeat analysis (MLVA) Genotyping of strains by variable number of tandem repeat (VNTR) traits was further genotyping based on serotyping to clarify the relationship between environmental strains and clinical strains , And has been widely used in the epidemic investigation of brucellosis. This paper summarizes the basic principle, development overview, superiority of MLVA technology and its application in the typing and traceability of Brucella.