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目的:建立一种基于免疫层析法(Immunochromatographic assay, ICA)的快速简便的人腺病毒(Human adenoviruses，HAdVs)检测方法。方法:利用抗原结合和病毒斑点实验检测发现单克隆抗体3C11和7E6能特异性结合所有测试的人腺病毒，以这两个抗体建立ICA方法。使用培养病毒及10份临床咽拭标本检测了该方法的特异性及灵敏性。结果:该ICA能特异性结合HAdV-1、2、3、4、5、6、7、10及胃肠型HAdV-41，极限范围为0.16～10n 3TCIDn 50/ml，所有成功分离出病毒的临床样本也显示阳性。n 结论:ICA检测方法是一种灵敏度高、特异性强、方便快速检测HAdVs的方法。“,”Objective:To establish a quick and convenient method for detecting human adenoviruses (Human adenoviruses, HAdV) based on immunochromatographic assay (ICA).Methods:Two antibody clones, 3C11 and 7E6 were found to bind to all tested HAdVs and then subsequently processed into ICA. The specificity and sensitivity were evaluated using representative strains of the respiratory HAdV types, including HAdV-1, 2, 3, 4, 5, 6, 7, 10 and a gastroenteric type HAdV-41 together with the original throat swabs of 10 HAdV patients confirmed by nuclear acid testing (NAT).Results:The ICA exhibited high specificity to HAdVs and its detection limitation ranged from 0.16 to 10n 3 half tissue culture infectious dose (TCIDn 50)/ml for different types of HAdVs. All clinic samples with successful virus isolation tested by this ICA showed positive result .n Conclusions:The ICA developed in the present study will be suitable for HAdVs screening in clinic setting, especially for those of respiratory types.