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目的 初步探讨一种新的无创伤的筛查膀胱癌的方法。 方法 收集 5 0例膀胱癌患者术前 (17例Ta T1,2 1例T2 ,10例T3 ,2例T4 ;11例Ⅰ级 ,2 3例Ⅱ级 ,16例Ⅲ级和 11例正常人尿液 ,采用Westernblot半定量方法检测其中基质金属蛋白酶 2 (MMP 2 )、MMP 9和组织基质金属蛋白酶抑制剂 2 (TIMP)的表达。 结果 (1)正常人尿液中无MMP 2、MMP 9的表达 ,而TIMP 2表达阳性率为9 0 9% ;(2 )膀胱癌患者尿液中MMP 2、MMP 9、TIMP 2的阳性表达率分别为 18% ,12 % ,80 % ,且MMP 9的阳性率表达与肿瘤的病理分期和分级具有相关性 ;(3)半定量分析 ,MMP 2、MMP 9表达强度与患者分期分级无关 ,而在Ⅲ级和T4 期患者尿液中TIMP 2表达平均强度具有高于Ⅰ Ⅱ级和Ta T3 期患者的趋势。 结论 MMP 9在膀胱癌的侵袭过程中起着重要作用 ,通过检测尿液中TIMP 2的表达 ,可为临床诊断膀胱癌提供一种新的无创的初筛方法
Objective To investigate a new noninvasive method of screening bladder cancer. Methods Fifty cases of bladder cancer were collected preoperatively (17 cases of Ta T1, T2 T2, 10 cases of T3, 2 cases of T4, 11 cases of Ⅰ, 23 cases of Ⅱ, 16 cases of Ⅲ and 11 cases of normal urine The expression of MMP 2, MMP 9 and tissue inhibitor of tissue matrix metalloproteinase 2 (TIMP) were detected by semi-quantitative Western blot.Results (1) There were no MMP 2, MMP 9 (2) The positive expression rates of MMP 2, MMP 9 and TIMP 2 in urine of bladder cancer patients were 18%, 12% and 80%, respectively, and the positive rates of MMP 9 (3) Semi-quantitative analysis showed that the expression of MMP-2 and MMP-9 was not correlated with the staging of patients, while the average intensity of TIMP-2 expression in urine of grade III and T4 patients With a higher tendency than those in patients with stage Ⅰ ¢ ò and stage ¢ ñTa.Conclusions MMP 9 plays an important role in the invasion of bladder cancer.It can provide a new clinical diagnosis of bladder cancer by detecting the expression of TIMP 2 in urine Non-invasive screening method