利用高效液相色谱反冲技术分析原油中胶质含量，是以键合相腈基柱为色谱柱，正庚烷为流动相，定量分析原油中胶质含量，以沉淀──重量法测定了在原油样品中的沥青质含量。在研究中，分别进行了色谱柱、流动相、反冲条件、检测器吸收波长等实验条件的选择与确定。并进行了色谱法与经典法对比实验及方法的回收年实验。同时给出了相对标准偏差和相对误差。 Using high-performance liquid chromatography backflushing technology to analyze the content of colloidal oil in crude oil, the phase of bonded nitrile-based column was used as the column, and n-heptane was used as the mobile phase to quantitatively analyze the content of colloidal oil in crude oil Asphaltene content in crude oil samples. In the study, the selection and determination of experimental conditions such as column, mobile phase, back-flushing conditions and detector absorption wavelength were carried out respectively. And the experiments of chromatography and classical comparison experiments and methods were carried out. At the same time, the relative standard deviation and relative error are given.