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成年雄性Ｗｉｓｔａｒ大鼠分为Ａ、Ｂ组在Ｔ_８～Ｔ_（１０）做脊髓半横断切损２．０ｍｍ；Ｃ、Ｄ组在Ｔ_８～Ｔ_（１０）做脊髓全横断切损２．０ｍｍ。Ａ、Ｃ组接受成体竖脊肌的移植体，Ｂ、Ｄ组接受胎鼠（Ｅ_（１８））股部肌肉的移植体。Ｅ组，为无移植体的脊髓完全横断对照组。术后２个月的ＣＴ－ＨＲＰ示踪及组织学（Ｇｌｅｅｓ）银染色方法显示，在各实验组动物的骨骼肌移植体与受体脊髓间呈不同程度的组织愈合，特别是在胎鼠骨胳肌移植的Ｂ组和Ｄ组，脊髓与肌肉移植体之间出现一段两种组织交错的融合区。在两种骨骼肌移植体内，都发现有再生的银染和ＣＴ－ＨＲＰ标记的阳性神经纤维沿着骨骼肌纤维生长行走。Ａ、Ｃ组自体移植体的骨骼肌横纹清晰可见；Ｂ、Ｄ组移植体的胎鼠骨骼肌组织已变性，横纹消失。在移植体两端与脊髓组织愈合处及肌组织内发现的神经纤维提示，再生的神经纤维可通过骨胳肌移植体进入脊髓远段，而且胎鼠骨胳肌移植体优于成体的骨骼肌移植。本文讨论了骨骼肌作为中枢神经移植体修复脊髓缺损的可能性，并推测骨骼肌移植体内可能存在诱导神经纤维再生的特异神经生长因子，促进了损伤脊髓的修复。
Adult male Wistar rats were divided into A and B groups. The hemisection of spinal cord was 2.0mm in T8 ~ T10 (10). The transection of the spinal cord was 2.0mm in T8 ~ T10 in C and D groups. Groups A and C received adult erector spinae grafts, while groups B and D received grafts of fetal (E 18) femoral muscles. Group E, a completely transected control group of non-graft spinal cord. CT-HRP tracing and Glees silver staining 2 months after surgery showed that there was some degree of tissue healing between the skeletal muscle grafts and the recipient spinal cord in each experimental group, especially in fetal rat bone In Group B and Group D of the skeletal muscle transplantation, there was a confluent area of fusion between the spinal cord and the muscle graft. In both skeletal muscle grafts, regenerative silver staining and CT-HRP-labeled positive nerve fibers were found to be found along skeletal muscle fiber growth. The skeletal muscle stripes of autograft in group A and group C were clearly visible. The skeletal muscle tissue of group B and group D had been degenerated, and the horizontal stripes disappeared. Nerve fibers found at both the graft and spinal cord tissue healing sites and in muscle tissue suggest that regenerated nerve fibers can enter the distal segment of the spinal cord through skeletal muscle grafts and that fetal rat skeletal muscle grafts outperform adult skeletal muscle transplant. This article discusses the possibility of using skeletal muscle as a central nerve graft to repair spinal cord defects and speculate that there may be specific nerve growth factor that can induce nerve fiber regeneration in skeletal muscle transplantation and promote the repair of injured spinal cord.