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用1～40Gy不同剂量γ线照射人外周血淋巴细胞,加PHA刺激剂培养淋巴细胞,制备含IL-2的上清标本,以观察内源性IL-2的动力学;加纯化IL-2制剂观察外源性IL-2对损伤细胞的效应。以CTLL。细胞测定IL-2的活性单性,用~3H-TdR掺入法测定细胞增殖和单克隆抗体检测T细胞亚群。实验结果表明:受照射的淋巴细胞DNA合成降低,但在1～10Gy范围内可被加入纯化IL-2而部分逆转。2.5Gy照射的淋巴细胞,各种T细胞亚群的数目均减少,CD_4~+/CD_8~+细胞比值升高,当加IL-2后,T细胞的数目和亚群比值均有所恢复。IL-2动力学显示,1～10Gy照射淋巴细胞,IL-2产量随照射剂量的加大而增加,大于10Gy时IL-2产量逐步下降,其峰值在10Gy。
Different doses of γ-rays from 1 to 40 Gy were used to irradiate human peripheral blood lymphocytes and lymphocytes were cultured with PHA stimulator to prepare supernatant samples containing IL-2 to observe the kinetics of endogenous IL-2; purified IL-2 was added. Preparations were observed for the effects of exogenous IL-2 on damaged cells. Take CTLL. Cells were assayed for IL-2 activity, cell proliferation was measured by ~3H-TdR incorporation, and monoclonal antibody detection was performed on T cell subsets. The experimental results show that the DNA synthesis of irradiated lymphocytes is reduced, but can be partially reversed by adding purified IL-2 in the range of 1 to 10 Gy. In 2.5Gy lymphocytes, the number of various T cell subpopulations decreased, and the ratio of CD4+/CD8+ cells increased. When IL-2 was added, the number of T cells and the ratio of subpopulations recovered. The kinetics of IL-2 showed that IL-1 production increased with irradiation dose from 1 to 10 Gy, and IL-2 production decreased gradually when the irradiation dose was greater than 10 Gy. The peak value was 10 Gy.