This paper chooses the case of The Big Bang Theory, employing Grice’s co-operative principle as the theoretical analysis tool, exploring the humorous effect through analyzing in depth and providing some inspirations for applying the Cooperative Principle. This paper chooses the case of The Big Bang Theory, employing Grice’s co-operative principle as the theoretical analysis tool, exploring the humorous effect through analyzing in depth and providing some inspirations for applying the Cooperative Principle.