论文部分内容阅读
目的 探讨犬用口服狂犬病减毒活疫苗（ＣＴＮ－ ２２ 毒株）的安全性。方法 依照ＷＨＯ专家会议关于犬和野生食肉动物口服狂犬病疫苗接种现场试验要求和标准的报告；以及美国动物与动物制品法规—兽医生物制品标准，进行本疫苗的安全性试验。结果 口服免疫的５ 只猫和口服、注射免疫的各５ 只黄毛鼠，观察２１天，无任何症状发生，均健康存活；用本疫苗１０个现场使用量的１ｍ ｌ，各对５ 只家犬肌肉和大隐神经及其周围组织浸润注射后，观察３～３５ 天，无狂犬病症状，并按方法中所规定时间淘汰犬，剖取颈淋巴结、脑、唾液腺等组织分离病毒，均为阴性；３ 月龄１０ 只犬以１０ 个现场剂量口服免疫后，１、２、３ 天连续３ 次取唾液进行病毒分离亦均阴性，免疫后观察６个月未显示狂犬病任何征象，均健康存活。结论 证实ＣＴＮ－ ２２毒株制备的犬用口服狂犬病减毒活疫苗是安全的
Objective To investigate the safety of oral attenuated live rabies vaccine (CTN-22 strain) in dogs. Methods The safety testing of this vaccine was conducted in accordance with the WHO Expert Meeting Report on Field Test Requirements and Criteria for Oral Rabies Vaccination in Canine and Wild Carnivores; and the U.S. Animals and Animal Products Legislation - Veterinary Bioproducts Standards. Results 5 cats orally immunized and 5 rhesus mice orally and immunized each day were observed for 21 days without any symptoms and all survived healthily. Canine and saphenous nerve and its surrounding tissues were infiltrated for 3 to 35 days without symptoms of rabies. The dogs were eliminated according to the time required by the method, and the cervix lymph node, brain, salivary gland and other tissues were dissected to isolate the virus, which were all negative ; 10 dogs at 3 months of age were orally immunized with 10 field doses, and 3 and 3 days after taking oral saliva respectively for 3 and 3 days were also negative. After 6 months of immunization, no signs of rabies were observed and all survived. Conclusion It is confirmed that the live attenuated rabies vaccine for dogs prepared from CTN-22 strain is safe