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目的 :观察C -反应蛋白 (CRP)和脂多糖 (LPS)诱导人外周血单核细胞合成白细胞介素 - 6水平及两者致单核细胞NF -κB活化的程度 ,比较其致炎作用。方法 :Ficoll密度梯度离心法分离人外周血单核细胞 ,应用酶联免疫吸附试验 (ELISA)法观察CRP和LPS刺激单核细胞产生IL - 6的时间效应 ,比较其峰值。免疫细胞化学观察两者致单核细胞NF -κB活化的程度。结果 :CRP和LPS刺激IL - 6合成开始的时间分别是 4小时和 2小时 ,呈时间依赖性 ,在 2 4小时达高峰。LPS组峰值高于CRP组。在刺激 1 6小时后 ,LPS组NF -κB活化的程度高于CRP组。结论 :CRP和LPS均能活化NF -κB ,并诱导单核细胞产生IL - 6 ;1 0ng/mlLPS的致炎作用强于 2 0ug/mlCRP。
Objective: To observe the levels of interleukin - 6 (IL - 6) induced by C - reactive protein (CRP) and lipopolysaccharide (LPS) in human peripheral blood mononuclear cells and the activation of NF - κB in both monocytes. Methods: Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation. The time effect of monocyte producing IL - 6 induced by CRP and LPS was observed by enzyme - linked immunosorbent assay (ELISA). Immunocytochemistry Observe the extent of both NF-κB activation by monocytes. Results: The time of IL - 6 synthesis stimulated by CRP and LPS was 4 hours and 2 hours, respectively, in a time - dependent manner, reaching a peak at 24 hours. The peak value of LPS group was higher than that of CRP group. After 16 hours of stimulation, NF-κB activation in LPS group was higher than that in CRP group. CONCLUSION: Both CRP and LPS can activate NF-κB and induce monocytes to produce IL - 6. The pro-inflammatory effect of 10 ng / ml LPS is stronger than that of 20 μg / ml CRP.