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目的探讨骨髓间充质干细胞(MSCs)对辐射诱发胸腺瘤过程中β-catenin和c-myc mRNA表达影响,为阐明MSCs在肿瘤发生发展中作用提供理论依据。方法全骨髓贴壁培养C57BL/6小鼠MSCs,X射线照射C57BL/6小鼠建立胸腺淋巴瘤模型,将小鼠随机分成对照组、辐射组及MSCs组,每组6只;逆转录-聚合酶链反应技术检测各组小鼠胸腺β-catenin和c-myc mRNA表达。结果辐射组小鼠胸腺瘤β-catenin mRNA表达量(0.92±0.23)明显高于对照组(0.64±0.21)(P<0.05),MSCs组β-catenin mRNA表达量(0.77±0.24)下降;与对照组(1.48±0.34)比较,辐射组c-myc mRNA表达量(1.61±0.37)上调,MSCs组c-myc mRNA表达量(1.18±0.34)明显低于辐射组(P<0.05)。结论 MSCs可通过抑制β-catenin和c-myc基因异常表达,抑制肿瘤的增殖。
Objective To investigate the effect of bone marrow mesenchymal stem cells (MSCs) on the expression of β-catenin and c-myc mRNA during radiation-induced thymoma and to provide a theoretical basis for elucidating the role of MSCs in tumorigenesis. Methods C57BL / 6 mouse MSCs were adherently cultured in whole bone marrow. Thymus lymphoma models were established by X-ray irradiation in C57BL / 6 mice. The mice were randomly divided into control group, radiation group and MSCs group, with 6 mice in each group. Reverse transcription- Enzyme-linked immunosorbent assay was used to detect the expression of β-catenin and c-myc mRNA in thymus of mice in each group. Results Compared with the control group (0.64 ± 0.21), the expression of β-catenin mRNA in the thymoma group (0.92 ± 0.23) in radiation group was significantly lower than that in the control group (0.77 ± 0.24) Compared with control group (1.48 ± 0.34), the expression of c-myc mRNA in radiation group was significantly higher than that in radiation group (1.61 ± 0.37) (1.18 ± 0.34) (P <0.05). Conclusion MSCs can inhibit tumor proliferation by inhibiting the abnormal expression of β-catenin and c-myc.