论文部分内容阅读
目的:研究IL-2对人垂体腺瘤细胞的增殖、细胞周期、细胞内蛋白激酶C(PKC)及cAMP/cGMP的影响,并探讨其作用的机制。方法:采用MTT比色法和3H-TdR掺入法检测IL-2对人垂体腺瘤细胞增殖和DNA合成的影响;用流式细胞术检测IL-2对垂体腺瘤细胞细胞周期的影响。用放射免疫测定法检测PKC的活性及cAMP和cGMP的含量。结果:(1)IL-2可促进垂体腺瘤细胞增殖和DNA合成,且呈剂量依赖性。(2)IL-2可显著降低垂体腺瘤细胞G1期的细胞比率,而增加S期和G2期的细胞比率。(3)与空白处理组相比较,使用PKC的激动剂PMA处理培养的人垂体腺瘤细胞,可使胞膜和细胞总PKC升高;而用IL-2(1×105U/L)处理后,胞质、胞膜和细胞总PKC的活性均升高。(4)IL-2(5×104、1×105U/L)作用于人垂体腺瘤细胞后,胞内cAMP浓度显著降低;而cGMP的浓度没有明显改变。结论:IL-2对垂体腺瘤细胞增殖分化的调控作用是细胞内多信息系统相互整合的结果。
Objective: To investigate the effect of IL-2 on the proliferation, cell cycle, intracellular protein kinase C (PKC) and cAMP / cGMP in human pituitary adenoma cells and to explore its mechanism. Methods: The effect of IL-2 on the proliferation and DNA synthesis of human pituitary adenoma cells was detected by MTT assay and 3H-TdR incorporation. The effect of IL-2 on the cell cycle of pituitary adenoma cells was detected by flow cytometry. The activity of PKC and the contents of cAMP and cGMP were detected by radioimmunoassay. Results: (1) IL-2 can promote the proliferation and DNA synthesis of pituitary adenoma cells in a dose-dependent manner. (2) IL-2 can significantly reduce the cell ratio of pituitary adenoma cells in G1 phase and increase the cell ratio in S phase and G2 phase. (3) Compared with the blank control group, PKC agonist PMA treatment of cultured human pituitary adenoma cells, can make the membrane and cell total PKC increased; with IL-2 (1 × 105U / L) after treatment , Cytoplasm, membrane and cell total PKC activity were increased. (4) After treated with IL-2 (5 × 104, 1 × 105U / L), the intracellular cAMP concentration decreased significantly; however, the concentration of cGMP did not change significantly. Conclusion: The regulatory effect of IL-2 on the proliferation and differentiation of pituitary adenoma cells is the result of the integration of multi-information system in cells.