论文部分内容阅读
目的研究少突胶质细胞前体细胞(OPC)的发育特点及围生期电生理特性。方法应用免疫组化及Western blot,观察生后不同发育阶段大鼠大脑皮质、海马等区域OPC细胞数量。应用脑片膜片钳记录出生后7d大鼠皮层白质和海马OPC电生理学特性。结果出生后0、7、14、21、35、50d、18个月大鼠大脑皮层及海马区域均有OPC细胞表达。在发育生后7d,细胞数量密度最高,成年大鼠也有较高数量OPC的分布。P7d大鼠海马OPC有小的内向Na+电流,而白质区域OPC仅表现外向K+电流和细胞膜被动反应。结论P7d大鼠皮层及海马区域OPC细胞数量及NG2蛋白表达量最大,海马和白质区域的OPC表现电生理学特性的异质性。
Objective To study the development characteristics of oligodendrocyte precursors (OPCs) and their perinatal electrophysiological characteristics. Methods Immunohistochemistry and Western blot were used to observe the numbers of OPCs in the cerebral cortex, hippocampus and other regions of postnatal rats at different developmental stages. The slices were used to record the electrophysiological characteristics of cortical white matter and hippocampus of rats 7 days after birth. Results OPC cells were expressed in the cerebral cortex and hippocampus of rats at 0, 7, 14, 21, 35, 50 and 18 months after birth. At 7 days after development, the number of cells was the highest, and adult rats also had a higher number of OPCs. P7d hippocampal OPC has a small inward Na + current, while white matter OPC only shows outward K + current and membrane passive response. Conclusions The number of OPCs and the expression of NG2 protein in the cortex and hippocampus of P7d rats are the largest. The OPC in the hippocampus and white matter shows the heterogeneity of electrophysiological characteristics.