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目的:及时有效监测银杏叶提取物柱层析洗脱液的生产过程,减少人员操作间的差异,提高生产效率。方法:利用近红外光谱在线监控技术监测银杏叶提取物柱层析洗脱液中总黄酮成分,并通过HPLC离线分析所得的多指标成分含量变化趋势图进行对比分析,验证可否用于生产状况实时监测,指导柱层析洗脱液收集起点和终点。结果:定量模型预测含量与液相色谱检测含量相关性良好,可以快速判断柱层析起点和终点。结论:该方法使柱层析生产操作简便、快捷,提高了生产效率。有利于产品质量的均一、稳定、可控。
OBJECTIVE: To monitor the production of column chromatography eluate in Ginkgo biloba extract timely and effectively, reduce the differences among personnel and improve the production efficiency. Methods: The content of total flavonoids in column chromatographic eluate of Ginkgo biloba extract was monitored by near infrared spectroscopy online monitoring technology, and the content trend of multi-index components was analyzed by HPLC offline analysis to verify whether it can be used in real-time production status Monitor, direct column chromatography eluent collection start and end points. Results: Quantitative model predictive content and liquid chromatography detection of a good correlation, you can quickly determine the start and end of column chromatography. Conclusion: This method makes the operation of column chromatography simple and quick, and improves the production efficiency. Conducive to product quality, stability, controllable.