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目的探讨B16F10黑素瘤细胞培养上清对相同基因小鼠腹腔巨噬细胞的抑制作用。方法制备B16F10黑素瘤细胞培养上清,并作用于相同基因小鼠腹腔巨噬细胞,经细菌脂多糖(LPS)刺激,通过中性红吞噬试验检测巨噬细胞吞噬功能和MTT法检测巨噬细胞活性。结果在B16F10黑素瘤细胞培养上清的作用下,LPS诱导的相同基因小鼠腹腔巨噬细胞吞噬中性红的能力为0.552±0.060,巨噬细胞活性为0.411±0.035,与对照组(1.247±0.164和0.861±0.208)相比,差异均有统计学意义(P均<0.005)。结论 B16F10黑素瘤细胞培养上清对相同基因小鼠腹腔巨噬细胞具有抑制作用。
Objective To investigate the inhibitory effect of B16F10 melanoma cell culture supernatant on murine peritoneal macrophages. Methods The supernatant of B16F10 melanoma cells was prepared and stimulated by bacterial lipopolysaccharide (LPS) in the same gene mouse peritoneal macrophages. Phagocytosis of macrophages was detected by neutral red phagocytosis assay and macrophage Cell activity. Results Under the action of B16F10 melanoma cell culture supernatant, the peritoneal macrophages induced by LPS had the ability of phagocytosis of neutral red at 0.552 ± 0.060 and the macrophage activity at 0.411 ± 0.035, which was significantly lower than that of the control group (1.247 ± 0.164 and 0.861 ± 0.208 respectively), the difference was statistically significant (all P <0.005). Conclusion B16F10 melanoma cell culture supernatant can inhibit peritoneal macrophages of the same gene in mice.