讨论了用微量热法测定酶促反应的米氏常数，酶活性，表观速率常数及表观活化能等动力学参数的实验方法和测定原理．介绍了摩尔反应焓的测定和计算方法，并借助过渡状态理论，探讨了酶底复合过程的吉氏自由能、焓和熵等热力学函数值的测定和计算方法 The experimental method and determination principle of the microcalorimetric determination of kinetic parameters such as Michaelis constant, enzyme activity, apparent rate constant and apparent activation energy were discussed. The methods for the determination and calculation of molar enthalpy of reaction are introduced. The determination and calculation of the thermodynamic function values ​​such as Gibbs free energy, enthalpy and entropy of the process of enzyme substrate recombination are discussed by means of the transition state theory